Introduction
Tonsil-derived mesenchymal stem cells (TMSCs) with capacities to differentiate into mesodermal, endodermal and ectodermal lineages have been wildly addressed for their practical applications in many disease animal models. TMSCs’ differential and proliferation potency may be different according to the donors (donor dependency). However, no one have compared the stemness of TMSCs from donors with different ages or gender, or TMSC isolated from different parts of tonsil. In this study, we characterized TMSCs isolated from epidermis and stroma of tonsil for their proliferation rate, multi-potency and surface marker profile in gender-dependent and age-dependent manners.
Methods
We divided tonsil tissue into epidermis, stroma and both parts. TMSCs isolated from these parts were classified into male and female donors and sub-classified into before, within and after puberty period groups. Then we characterized TMSCs for their proliferation, multi-potency differentiation into adipocytes, osteocytes, chondrocytes and surface protein profile.
Results
Our data showed that TMSCs isolated from young donors grew faster than ones from old donors. Between male and female donors, TMSCs from male had higher proliferation than female. Within sub-classes both, epidermis and stroma, TMSCs from stroma showed the highest proliferation rate. Besides, the adipogenic potential of stroma-TMSCs was also higher than both or epidermis-TMSCs. Osteogenesis, chondrogenesis and surface protein profile of TMSCs will be investigated.
Conclusion
Our data suggested that donor age, gender and tissue niches can influence the quality and characteristics of TMSCs. Optimization these conditions might help giving rise to a more stable TMSC source for therapeutic applications in the future.