Poster Presentation ASSCR, AGCTS, ISCT ANZ and Friends Joint Scientific Conference 2019

Generation of human-induced pluripotent stem cell lines from patients with spontaneous coronary artery dissection  (#136)

Keerat Junday 1 , Monique Bax 1 , Melanie Lim 1 , Ketan Mishra 1 , Claire Wong 1 , Andrea Chan 1 , Stephanie Hesselson 1 , David Muller 1 , Siiri Iismaa 1 , Ashish Mehta 1 , Robert Graham 1
  1. Victor Chang Cardiac Research Institute, Sydney, NSW, Australia

Spontaneous coronary artery dissection (SCAD) is a non-atherosclerotic form of coronary artery disease of unknown cause that predominantly affects women (>90%; mean age 44-55 years) and can be fatal. The finding of familial clustering, including the concordant involvement of monozygotic twins and its association with the PHACTR1/EDN1 genetic locus, indicates a genetic predisposition to its pathophysiology. Human-induced pluripotent stem cell lines (hiPSCs) were generated from patients who had survived an episode of SCAD. The cells generated using non-integrative Sendai virus and show expression of all pluripotency markers, the ability to differentiate into three germ layers and are genetically stable. These disease-specific hiPSC lines along with their healthy control cell lines will be useful for the study of SCAD after differentiation into blood vessel-forming cells.

 

  • Have you presented your abstract at another international meeting?: No