Poster Presentation ASSCR, AGCTS, ISCT ANZ and Friends Joint Scientific Conference 2019

Transcriptomic data of human mesenchymal stem cells secretome and targets for wound healing (#214)

Huan Ting Ong 1 2 , Sharon Redmond 1 , Dulce B Vargas-Landin 3 4 , Alistair Forrest 3 , Rodney Dilley 1 5
  1. Ear Science Institute Australia, Subiaco, WA, Australia
  2. Faculty of Health and Medical Sciences, The University of Western Australia, Nedlands, WA, Australia
  3. Harry Perkins Institute of Medical Research, Nedlands, WA, Australia
  4. School of Molecular Sciences, The University of Western Australia, Nedlands, WA, Australia
  5. The Ear Sciences Centre and Center for Cell therapy and Regenerative medicine, The University of Western Australia, Nedlands, Western Australia, Australia

Our previous studies showed human adipose-derived mesenchymal stem cells (ADSCs) produce an activity that stimulated wound healing in eardrum keratinocytes (hTMk)1. By regulating ADSCs in hypoxia (<0.1% O2), this wound healing stimulus was increased. To understand the molecular mechanism behind this paracrine activity of ADSCs on wound healing, we used a bioinformatics approach to determine ADSCs and hTMk transcriptome for secreted ligands and receptors. Primary ADSCs were cultured in ambient oxygen conditions (21%) or hypoxia for 48h without serum. hTMk were established under normoxic conditions. Conditioned media were collected from ADSCs to assess paracrine activity on hTMk proliferation and migration, and to quantify specific protein secretion using ELISA. Transcriptomic analysis of ADSCs and hTMk were assessed using RNAseq and bioinformatics. Transcripts differentially expressed (P-adj <0.05) between each primary hypoxic and normoxic ADSCs were filtered through databases to identify secreted ligands, and similarly for hTMk transcriptome to identify receptors. Both subsets were then matched to a FANTOM5 curated ligand-receptor pair database2 to establish the final list of ligand-receptor repertoires. Results show hypoxia in ADSC consistently up-regulated VEGFA more than 3 fold, with corresponding receptors expressed at mRNA level on the hTMk. This could potentially represent a previously unknown function in wound healing.

  1. Ong H.T., Redmond S.L., Marano R.J., Atlas M.D., von Unge M., Aabel P. and Dilley R.J. Paracrine Activity from Adipose-Derived Stem Cells on In Vitro Wound Healing in Human Tympanic Membrane Keratinocytes. 2017. Stem Cells and Development. 26(6): 405-418
  2. Ramilowski J.A., Goldberg T., Harshbarger J., Kloppman E., Lizio M., Satagopam V.P., Itoh M., Kawaji H., Carninci P., Rost B. and Forrest A.R.R. A Draft Network of Ligand-receptor-mediated Multicellular Signalling in Human. 2015. Nature Communications. 6:7866. DOI: 10.1038/ncomms8866
  • Have you presented your abstract at another international meeting?: Yes