Allowing scientists to research human genome editing technology is an issue that needs to involve both public stakeholders and policymakers. In this study we engaged the Australian public to express their opinions on this issue by delivering a series of 2 h educational workshops to members of University of the Third Age and Probus Australia; additionally at a Q & A forum during Sydney Science Festival. A questionnaire was distributed before and after the event. Of the 209 attendees, 75% completed the questionnaire. Majority of respondents were female (77%), aged over 60 yrs (66%), but a quarter were under 30 yrs (22%). Most (75%) identified with religion but half (51%) of these did not regularly attend a religious service. A quarter (23%) completed their education in high school but most (48%) had attended university. The majority (63%) were born in Australia. A quarter worked in medical or scientific areas, and a further 17% in corporate or professional jobs; 16% were retired. Majority (56%) had limited knowledge of gene editing before the educational event, but this changed after it (12%). 37% agreed with genome editing to allow heritable changes for health purposes before, increasing to 59% afterward. Those identifying with religion and additionally those with less formal education were less likely to change their views. Before the event 34% of respondents agreed with genome editing of human embryos for research, increasing to 63% afterward. Agreement with genome editing to treat disease as a part of a clinical trial increased from 57% before to 72% afterwards. Majority (84%) of all respondents disagreed with genome editing for non-health purposes before, with no change after. However, men were more likely to change their views. Findings from this study will assist in future science communication plans and policy development on human genome editing.